Flavin Mononucleotide-Based Fluorescent Proteins Function in Mammalian Cells without Oxygen Requirement
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چکیده
منابع مشابه
Flavin Mononucleotide-Based Fluorescent Proteins Function in Mammalian Cells without Oxygen Requirement
Usage of the enhanced green fluorescent protein (eGFP) in living mammalian cells is limited to aerobic conditions due to requirement of oxygen during chromophore formation. Since many diseases or disease models are associated with acute or chronic hypoxia, eGFP-labeling of structures of interest in experimental studies might be unreliable leading to biased results. Thus, a chromophore yielding ...
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reaction coordinate, but as a second dimension the distance between ions needs to be taken into account as ions do not move independently through the pore. The biasing potential in both equilibrium and nonequilibrium methods can be chosen arbitrarily, rendering the PMF approach suitable also for high barriers that are precluded from direct flux estimates, as the spontaneous permeation rate woul...
متن کاملFlavin-based fluorescent proteins: emerging paradigms in biological imaging.
Flavin-based fluorescent proteins (FbFPs) are an emerging class of fluorescent reporters characterized by oxygen-independent fluorescence and a small size - key advantages compared to the green fluorescent protein (GFP). FbFPs are at a nascent stage of development. However, they have already been used as versatile reporters for studying anaerobic biosystems and viral assemblies. Recently, FbFPs...
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The binding and function of FMN, 3,6,7-trimethyl-9(l’-o-ribityl)isoahoxazine 5’-phosphate (3-methyl-FMN), 6,7dichloro-9-(l’-o-ribityl)isoalloxazine 5’-phosphate (6,7-dichloro-FMN), and 6,7-dimethyl-9-(5’-hydroxypentyl)isoalloxazine 5’-phosphate (5’-hydroxypentyl-FMN) has been investigated with reduced nicotinamide adenine dinucleotide phosphate dehydrogenase and NADPH cytochrome c reductase fro...
متن کاملA biomimetic redox flow battery based on flavin mononucleotide
The versatility in design of redox flow batteries makes them apt to efficiently store energy in large-scale applications at low cost. The discovery of inexpensive organic electroactive materials for use in aqueous flow battery electrolytes is highly attractive, but is thus far limited. Here we report on a flow battery using an aqueous electrolyte based on the sodium salt of flavin mononucleotid...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0043921